Anti-RAB11FIP1 antibody produced in rabbit
SIGMA/HPA023904 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab1
Synonym: Anti-Rab-coupling protein; Anti-Rab11 family-interacting protein 1; Anti-Rab11-FIP1
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human lung and skeletal muscle tissues using HPA023904 antibody. Corresponding RAB11FIP1 RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunohistochemical staining of human Lung shows moderate granular cytoplasmic positivity in macrophages.
Immunohistochemistry Immunohistochemical staining of human Cerebellum shows strong granular cytoplasmic positivity in Purkinje cells.
Immunohistochemistry Immunohistochemical staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells and cells in seminiferous ducts.
Immunohistochemistry Immunohistochemical staining of human Duodenum shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle shows very weak granular cytoplasmic positivity in myocytes.
Immunofluorescence Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | TGSAKHRLHPVKPMNAMATKVANCS |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 0.25-2 μg/mL |
| immunohistochemistry: 1:50-1:200 | |
| UniProt accession no. | Q6WKZ4  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | Rab11-family interacting protein 1 (Rab11-FIP1) along with Rab11a regulates plasma membrane recycling. Human breast cancer cell lines show elevated expression of Rab11-FIP1 gene. Rab11-FIP1 controls adiponectin trafficking and release from the adipocyte.Rab11-FIP1 expression increases with increasing body mass index (BMI) of humans. Cell-based assay states that Rab11-FIP1A as a potential oncogene plays a role in cell cycle and apoptosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Rab11-family interacting protein 1 (Rab11-FIP1) is encoded by a gene mapped to human chromosome 8p11. The encoded protein is characterized with an amphipathic a-helix at carboxyl-terminal end involved in binding Rab11a. Rab11-FIP1 and Rab11a, along with other proteins, localize on plasma membrane recycling system vesicles in both non-polarized HeLa cells and polarized madin-darby canine kidney cells (MDCK). |
| Immunogen: | Rab11 family-interacting protein 1 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST76310 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |