Anti-PHF13 antibody produced in rabbit
SIGMA/HPA026830 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-PHD finger protein 13
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of human testis shows strong nuclear positivity in subset of cells in seminiferous ducts.
Western Blotting Western blot analysis in control (vector only transfected HEK293T lysate) and PHF13 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406961).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | GKRTIVRQGKQVVFRDEDSTGNDED |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunohistochemistry: 1:20-1:50 | |
| UniProt accession no. | Q86YI8  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | PHD finger protein 13 (PHF13) or survival time associated PHD finger protein in ovarian cancer 1 (SPOC1) is a tightly regulated chromatin-associated protein, involved in chromatin structure modulation. The encoded protein helps in proper mitotic chromosome condensation and cell division. PHF13 /SPOC1 might have a role in cell proliferation and oncogenesis. SPOC1 is crucial for spermatogonial stem cell differentiation and spermatogenesis. Elevated expression of SPOC1 leads to unresectable carcinomas and decreased survival rates in ovarian cancer patients. SPOC1/ PHF13 plays a vital role in radiosensitivity and DNA damage repair with the help of chromatin modifiers and DDR (DNA damage response) regulators. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | The gene PHD finger protein 13 (PHF13), also known as survival time associated PHD finger protein in Ovarian Cancer 1 (SPOC1), is mapped to human chromosome 1p36.3, a region associated with chromosomal instability and tumor development.s The gene codes for a 34kDa nuclear protein containing 300 amino acids. The protein is characterized with a C-terminal single PHD (plant homeodomain) involved in chromatin specific interactions and a bipartite nuclear localization sequence. PHF13 mRNA is ubiquitously expressed at a low level in all tissues and it is expressed at high level in the testis and ovaries. |
| Immunogen: | PHD finger protein 13 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST72593 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |