Anti-HTRA2 antibody produced in rabbit
SIGMA/HPA027366 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-High temperature requirement protein A2; Anti-HtrA2; Anti-Omi stress-regulated endoprotease; Anti-Serine protease 25; Anti-Serine protease HTRA2, mitochondrial; Anti-Serine proteinase OMI
Product Type: Chemical
By Recombinant Expression Western blot analysis in control (vector only transfected HEK293T lysate) and HTRA2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408049).
Immunohistochemistry Immunohistochemical staining of human duodenum shows strong cytoplasm granular positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | MAAPRAGRGAGWSLRAWRALGGIRW |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunohistochemistry: 1:200-1:500 | |
| UniProt accession no. | O43464  |
| Application: | All Prestige Antibodies®Powered by Atlas Antibodies is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. Anti-HTRA2 antibody produced in rabbit has been used in western blotting. |
| Biochem/physiol Actions: | HTRA2 (high temperature requirement protein A2) is a mitochondrial serine protease and is associated with various neurodegenerative disorders. HTRA proteases recognize the exposed hydrophobic areas in proteins and are responsible for their degradation. It helps in mitochondrial homeostasis and plays a proapoptotic role in the cytoplasm. It is also involved in the development of cancer. It might be associated with various cancers due to its apoptotic function. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | The gene HTRA2 (high temperature requirement protein A2) is mapped to human chromosome 2p13. The encoded protein has a PDZ (postsynaptic density of 95 kDa, disc large and zonula occludens 1) domain and a protease domain. It is mainly present in the mitochondrial intermembrane space and goes to the cytoplasm in presence of cellular stress. |
| Immunogen: | Serine protease HTRA2, mitochondrial Precursor recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST70804 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |