Anti-ELL3 antibody produced in rabbit
SIGMA/HPA028938 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-Elongation factor rna polymerase ii-like 3; Anti-Flj22637
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of human Testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry Immunohistochemical staining of human Lymph node shows strong nuclear and cytoplasmic positivity in germinal center cells.
Immunohistochemistry Immunohistochemical staining of human Prostate shows no positivity in glandular cells as expected.
Immunohistochemistry Immunohistochemical staining of human Skeletal muscle shows no positivity in myocytes as expected.
Immunofluorescence Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | HRGYLRLPGPGWSCLFSFIVSQCCQ |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 0.25-2 μg/mL |
| immunohistochemistry: 1:50-1:200 | |
| UniProt accession no. | Q9HB65  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | Elongation factor for RNA polymerase II 3 (ELL3) regulates elongation step in transcription. ELL3 modulates themitogen-activated protein kinase (ERK1/2) signaling pathway, favoring tumorigenesis and drug resistance in breast cancer. It is upregulated in mature and activated human B cells and is crucial for B cell lymphoma survival. ELL3 exists as transcriptional complex with estrogen receptor alpha (ERα), T-cell-specific transcription factor (GATA-3) and forkhead box protein A1 (FOXA1) and modulates interleukin-20 (IL-20) expression in breast cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Elongation factor for RNA polymerase II 3 (ELL3) is specific for testis and belongs to eleven nineteen lysine-rich leukemia (ELL) protein family. It encodes a 50 kDa protein, with a catalytic domain in N-terminus. The C-terminal domain of ELL3 is homologous to ELL. ELL3 gene is mapped to human chromosome 15q15.3. |
| Immunogen: | elongation factor RNA polymerase II-like 3 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST70913 |
| Physical form: | Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |