Anti-TKT antibody produced in rabbit
SIGMA/HPA029481 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, ab2
Synonym: Anti-transketolase
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human bone marrow, cerebral cortex, skeletal muscle and testis using Anti-TKT antibody HPA029481 (A) shows similar protein distribution across tissues to independent antibody HPA029480 (B).
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human bone marrow and skeletal muscle tissues using Anti-TKT antibody. Corresponding TKT RNA-seq data are presented for the same tissues.
Enhanced Validation-RNAi Knockdown Western blot analysis in A-549 cells transfected with control siRNA, target specific siRNA probe no. 1, using Anti-TKT antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Enhanced Validation-Orthogonal RNAseq Confirmation Western blot analysis in human cell lines A-549 and U2OS using Anti-TKT antibody. Corresponding TKT RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunohistochemistry Immunohistochemical staining of human bone marrow shows high expression.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry Immunohistochemical staining of human bone marrow using Anti-TKT antibody HPA029481.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex using Anti-TKT antibody HPA029481.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle using Anti-TKT antibody HPA029481.
Immunohistochemistry Immunohistochemical staining of human testis using Anti-TKT antibody HPA029481.
Immunofluorescence Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Western Blotting Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq independent RNAi knockdown Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | IAKTFKGRGITGVEDKESWHGKPLP |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:2500-1:5000 | |
| UniProt accession no. | P29401  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Application: | Anti-TKT has been used in immunohistochemistry and immunofluorescence. |
| Biochem/physiol Actions: | Transketolase (TKT) is essential for cell division, NADPH and nucleic acid synthesis. It plays an important role in redox homeostasis in cancer development. Suppression of TKT terminates HCC (hepatocellular carcinoma) growth in vivo. It has the ability to affect the NAPDH production to counteract oxidative stress. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Transketolase (TKT) is an important thiamine-dependent enzyme in the PPP (pentose phosphate pathway). It is located on chromosome 3p21. |
| Immunogen: | transketolase recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST75164 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |