Anti-SLC2A1 antibody produced in rabbit
SIGMA/HPA031345 - affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-DYT18; Anti-GLUT; Anti-GLUT1; Anti-HTLVR
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human placenta and testis tissues using HPA031345 antibody. Corresponding SLC2A1 RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.
Immunohistochemistry Immunohistochemical staining of human skin shows moderate membranous positivity in epidermal cells.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows strong membranous positivity in endothelial cells.
Immunohistochemistry Immunofluorescence staining of mouse brain shows moderate positivity in endothelial cells in the cerebral cortex.
Immunohistochemistry Immunohistochemical staining of human testis shows no membranous positivity in cells in seminiferous ducts as expected.
Immunofluorescence Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | GRTFDEIASGFRQGGASQSDKTPEE |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 0.25-2 μg/mL |
| immunohistochemistry: 1:1000-1:2500 | |
| UniProt accession no. | P11166  |
| Application: | Anti-SLC2A1 antibody produced in rabbit has been used in immunohistochemistry. |
| Biochem/physiol Actions: | The SLC2A1 (solute carrier family 2 member 1) gene, also referred to as GLUT1, encodes protein that is mainly involved in the transport of D-glucose across the blood-brain barrier. Mutation in this gene causes Glut-1 deficiency syndrome, which is characterized by reduced cerebrospinal fluid glucose concentrations (hypoglycorrhachia) and reduced erythrocyte glucose transporter activities in the patients along with infantile seizures, acquired microcephaly and developmental delay. It has been found to be critical in the development of the blood-brain barrier. Mutation in this gene has also been associated with paroxysmal exercise-induced dyskinesia and epilepsy. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | The SLC2A1 (solute carrier family 2 member 1) gene is 35kb in length and is mapped to human chromosome 1p35 → p31.3. The gene contains 10 exons and nine introns. Its mRNA is found to be expressed in several tissues. It is highly expressed in cerebral endothelial cells and the encoded protein has 12 transmembrane domains. |
| Immunogen: | solute carrier family 2 (facilitated glucose transporter), member 1 |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST87043 |
| Physical form: | Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |