Anti-METTL14 antibody produced in rabbit
SIGMA/HPA038002 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-KIAA1627; Anti-
Product Type: Chemical
Western Blotting JOURNAL CITATION: N(6)-methyladenosine of HIV-1 RNA regulates viral infection and HIV-1 Gag protein expression. By: Tirumuru, N., Zhao, B. S., et al. in Elife, 2016. PubMed ID: 27371828 Image collected and cropped by CiteAb from the following publication, (https://elifesciences.org/articles/15528), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human kidney shows moderate to strong nuclear positivity in glomeruli and cells in tubules.
Immunofluorescence Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | RSWNMDSRLQEIRERQKLRRQLLAQ |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 0.25-2 μg/mL |
| immunohistochemistry: 1:1000-1:2500 | |
| UniProt accession no. | Q9HCE5  |
| Application: | All Prestige Antibodies®Powered by Atlas Antibodies is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. Anti-METTL14 antibody produced in rabbit has been used in western blotting and immunofluorescence. |
| Application: | Anti-METTL14 antibody produced in rabbit has been used in: • cross-linking and RNA immunoprecipitation (CLIP) • western blot • immunohistochemical analysis |
| Biochem/physiol Actions: | METTL14 (methyltransferase like 14) is mainly responsible for m6A (N6-methyladenosine) RNA methylation. It forms a heterodimer with METTL3 and the complex catalyzes m6A RNA methylation. |
| Biochem/physiol Actions: | METTL14 mutation reduces m6A methylation in ~70% of endometrial tumors. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | The gene METTL14 (methyltransferase like 14) is mapped to human chromosome 4q. It is a homologue of METTL3. The protein has an N-terminal α-helical motif (NHM), methyltransferase domain and a C-terminal motif. |
| Immunogen: | methyltransferase like 14 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST79779 |
| Physical form: | Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |