Anti-UQCRFS1 antibody produced in rabbit
SIGMA/HPA041863 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-Rip1; Anti-Ris1; Anti-Risp; Anti-
MDL Number: MFCD01633826
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human heart muscle and pancreas tissues using HPA041863 antibody. Corresponding UQCRFS1 RNA-seq data are presented for the same tissues.
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human colon, kidney, liver and testis using Anti-UQCRFS1 antibody HPA041863 (A) shows similar protein distribution across tissues to independent antibody HPA050339 (B).
Enhanced Validation-By Independent Antibodies Western blot analysis using Anti-UQCRFS1 antibody HPA041863 (A) shows similar pattern to independent antibody HPA050339 (B).
Immunohistochemistry Immunohistochemical staining of human heart muscle shows moderate to strong cytoplasmic positivity in myocytes.
Immunohistochemistry Immunohistochemical staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry Immunohistochemical staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts and in Leydig cells.
Immunohistochemistry Immunohistochemical staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry Immunohistochemical staining of human colon using Anti-UQCRFS1 antibody HPA041863.
Immunohistochemistry Immunohistochemical staining of human kidney using Anti-UQCRFS1 antibody HPA041863.
Immunohistochemistry Immunohistochemical staining of human liver using Anti-UQCRFS1 antibody HPA041863.
Immunohistochemistry Immunohistochemical staining of human testis using Anti-UQCRFS1 antibody HPA041863.
Immunofluorescence Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
Western Blotting Western blot analysis in human cell line A-549.
Western Blotting Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | RVKKPEWVILIGVCTHLGCVPIANA |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:50-1:200 | |
| UniProt accession no. | P47985  |
| Application: | Anti-UQCRFS1 antibody produced in rabbit has been used in western blot analysis. |
| Biochem/physiol Actions: | Ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 (UQCRFS1) is a main component of cytochrome bc1 complex (complex III) in the mitochondria. It plays a vital role in redox reactions in the mitochondria as it is involved in electron transport. Elevated expression of UQCRFS1 is associated with the development of acute myeloid leukemia (ALL), ovarian cancer and breast cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 (UQCRFS1) gene is mapped to human chromosome 19q12. The encoded protein is mainly localized to inner membranes of mitochondria. |
| Immunogen: | ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST82804 |
| Physical form: | Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |