Anti-PLIN4 antibody produced in rabbit
SIGMA/HPA044682 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-KIAA1881; Anti-S3-12; Anti-perilipin 4
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human adipose tissue, breast, colon and liver using Anti-PLIN4 antibody HPA044682 (A) shows similar protein distribution across tissues to independent antibody HPA054657 (B).
Immunohistochemistry Immunohistochemical staining of human adipose tissue shows moderate membranous positivity in adipocytes.
Immunohistochemistry Immunohistochemical staining of human breast shows moderate membranous positivity in adipocytes.
Immunohistochemistry Immunohistochemical staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry Immunohistochemical staining of human colon shows no positivity in glandular cells as expected.
Immunofluorescence Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane, cytosol and lipid droplets.
Western Blotting Western blot analysis in human adipose tissue tissue.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | GVDTSKTVLTGTKDTVCSGVTGAMN |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 0.25-2 μg/mL |
| immunohistochemistry: 1:2500-1:5000 | |
| western blot: 0.04-0.4 μg/mL | |
| UniProt accession no. | Q96Q06  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) |
| Biochem/physiol Actions: | Perilipin 4 (PLIN4) might be involved in the formation of lipid droplets (LDs) in adipocytes. Perilipins play a vital role in regulation of lipid turnover. In mice, knockdown of this gene downregulates Plin5 expression and decreases cardiac lipid accumulation. Mutation in the gene is linked to development of gastric cancer. In addition, variation in the gene might also lead to obesity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Perilipin 4 (PLIN4) is a lipid droplet protein, encoded by the gene mapped to human chromosome 19q13.3-13.4. PLIN4 is highly expressed in white adipose tissue and is also expressed at low levels in heart and skeletal muscle. The encoded protein and PLIN5 exhibit distinct though overlapping tissue expression pattern. |
| Immunogen: | perilipin 4 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST82778 |
| Physical form: | Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |