Bouin’s solution
SIGMA/HT101128 - histological fixative
Synonym: Bouin’s fluid
MDL Number: MFCD00146169
Product Type: Chemical
Staining image is the result of Procedure No. HT10.
| application(s) | hematology histology |
| color | yellow |
| composition | acetic acid, 5% |
| formaldehyde, 9% | |
| picric acid, 0.9% | |
| Quality Level | 200  |
| shelf life | Expiry date on the label |
| storage temp. | room temp |
| Application: | Bouin′s solution has been used: • in the staining of human liver biopsy tissue samples for two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging • in the fixation of rat ovaries for the observing follicle cells using microscopy • in the fixation of gonads of crab for morphological and histological analysis |
| Application: | Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures. |
| General description: | Bouin′s solution is a noncoagulant yellow stain fixative. It imposes shrinkage on tissues when used as preservative due to its picric acid component. It is majorly used in ureteroscopic biopsies. It preserves morphological and nuclear information in tissues upon fixation. |
| Symbol |   GHS06,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H317 - H319 - H331 - H335 - H341 - H350 |
| Precautionary statements | P280 - P301 + P312 - P302 + P352 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | E,T,Xn |
| Risk Statements | 45-1-3-20/21/22-36/37/38-40-41-43-68 |
| Safety Statements | 53-26-35-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |