Bouin’s solution
SIGMA/HT10132 - histological fixative
Synonym: Bouin’s fluid
MDL Number: MFCD00146169
Product Type: Chemical
Staining image is the result of Procedure No. HT10.
| application(s) | hematology histology |
| color | yellow |
| composition | acetic acid, 5% |
| formaldehyde, 9% | |
| picric acid, 0.9% | |
| Quality Level | 200  |
| shelf life | Expiry date on the label |
| storage temp. | room temp |
| Application: | Bouin′s solution is an excellent fixative for preserving soft and delicate structures. It is used as a mordant in various trichrome procedures. Additionally, it has been used for the following purposes- • staining of human liver biopsy tissue samples for two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging • fixation of rat ovaries for the observing follicle cells using microscopy • fixation of gonads of crab for morphological and histological analysis |
| Application: | Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures. |
| Biochem/physiol Actions: | Bouin′s solution causes shrinkage of tissues when used as a preservative due to its picric acid component. It preserves morphological and nuclear information in tissues upon fixation. |
| General description: | Bouin′s solution is a noncoagulant yellow stain fixative that consists of saturated picric acid, formaldehyde, and acetic acid. |
| Symbol |   GHS06,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H317 - H319 - H331 - H335 - H341 - H350 |
| Precautionary statements | P280 - P301 + P312 - P302 + P352 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | E,T,Xn |
| Risk Statements | 45-1-3-20/21/22-36/37/38-40-43-68 |
| Safety Statements | 53-26-35-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |