Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM) antibody produced in mouse
SIGMA/I0138 - clone RG-16, ascites fluid
Synonym: Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
Product Type: Chemical
ELISA 1:40,000 Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM) antibody produced in Mouse, Clone: RG-16 (Cat. No. I0138) followed by 1:500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was probed to detect Rabbit Immunoglobulins by indirect ELISA. Reactivity is observed with Rabbit IgG, Rabbit IgA and Rabbit IgM but not with Human IgG, Human IgA and Human IgM.
| antibody form | ascites fluid |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | RG-16, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| shipped in | dry ice |
| should not react with | pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:10,000 |
| Application: | Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM) antibody produced in mouse has been used in enzyme linked immunosorbent assay (ELISA), immunoblots, dot blots and immunohistochemistry. |
| Application: | The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature. |
| Biochem/physiol Actions: | Binds only rabbit Igs. Does not react with reduced rabbit Igs. |
| Biochem/physiol Actions: | Immunoglobulins acts as a cell-surface receptor for antigen that allows cell signaling and cell activation. In addition, it also acts as a soluble effector molecule that can recognize, bind and neutralize antigens. IgG contributes to immune response including neutralization of toxins and viruses. IgM provides first line of defense and is involved in immunoregulation. IgA protects mucous membranes against toxins, virus and bacteria by direct neutralization or by prevention of binding to the mucosal membranes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Rabbit Immunoglobulins (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins are the members of the eponymous immunoglobulin super-family (IgSF). Immunoglobulins are heterodimeric proteins characterized with two heavy (H) and two light (L) chains. Based on heavy chain C domains immunoglobulins are classified in to five main classes namely IgM, IgG, IgA, IgD, and IgE isotypes. |
| General description: | Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |