Anti-Human IgM (μ-chain specific) antibody produced in rabbit
SIGMA/I0140 - IgG fraction of antiserum, buffered aqueous solution
MDL Number: MFCD00162459
Product Type: Chemical
ELISA
Anti-Human IgM (μ-chain specific) antibody produced in Rabbit (Cat. No. I0140), followed by 1:5,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545), was serially diluted to detect Human IgM by indirect ELISA.
Anti-Human IgM (μ-chain specific) antibody produced in Rabbit (Cat. No. I0140), followed by 1:5,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545), was serially diluted to detect Human IgM by indirect ELISA.
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 300  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:100,000 |
| Application: | Anti-Human IgM (μ-chain specific) antibody produced in rabbit was used as secondary antibody in immunoblotting and indirect ELISA. |
| Biochem/physiol Actions: | IgM antibodies are present as pentamers in the serum and are produced in response to antigens. |
| Biochem/physiol Actions: | Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM is an initial immunoglobulin produced in the developing fetus as a result of the immune response during the second trimester of pregnancy. It is then substituted by IgG. Therefore, the diagnosis of specific antibodies of the IgM class is useful in detecting chronic infections. IgM is essential for the fetal and neonatal host defense system. IgM cannot cross the placenta. Hence, the presence of IgM might indicate an intrauterine viral infection. IgM antibodies are responsible for the agglutination of red blood cells in mismatched blood transfusions. The level of IgM may vary with the status of disease or infection. |
| Biochem/physiol Actions: | The antibody preparation is specific for human IgM when tested against purified human IgA, IgG, IgM, Bence Jones kappa, and lambda myeloma proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgM is a class of antibodies containing a pentamer of five IgG equivalents with 10 Fab fragments, joined by a single cross-linking peptide (J-chain). It constitutes 15% of the normal adult immunoglobulins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots at –20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |