Anti-α-Internexin antibody, Mouse monoclonal
SIGMA/I0282 - clone 2E3, purified from hybridoma cell culture
Synonym: Anti-66 kDa Neurofilament Subunit; Anti-INA; Anti-NF-66
MDL Number: MFCD01322222
Product Type: Chemical
Western Blotting Rat brain cytosolic extract were separated on SDS-PAGE and probed with Anti-α-Internexin antibody, Mouse monoclonal, Clone: 2E3 (Cat. No. I0282). The antibody was developed using 1:50,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E3, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~66 kDa by SDS-PAGE |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | feline, rat, bovine, human, pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.5 μg/mL using rat brain cytosolic extract | |
| UniProt accession no. | Q16352  |
| Application: | Anti-α-Internexin antibody, Mouse monoclonal has been used in: • enzyme linked immunosorbent assay (ELISA) • immunoblotting • immunohistochemistry • immunocytochemistry |
| Biochem/physiol Actions: | The expression of α-Internexin precedes the expression of NF triplet proteins during the differentiation of neurons. Its expression is relatively low when compared to the the NF proteins in the adult brain. It co-assembles with the NF triplet proteins. Overexpression of α-internexin in transgenic mouse model has been found to cause abnormal neurofilamentous accumulations and motor coordination deficits. It has been found to be present in cytoplasmic inclusions linking it to NIFID (neuronal intermediate filament inclusion disease) and other neurological diseases with pathological accumulations of IFs (intermediate filaments). α-Internexin may be useful as a biomarker for pancreatic neuroendocrine tumor aggressiveness and prognosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-α -Internexin (mouse IgG1 isotype) is derived from the 2E3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with the full-length recombinant rat α -internexin. |
| General description: | The gene INA (α-Internexin) encodes a neuronal intermediate filament (IF) protein that is a type IV IF. The gene is mapped to human chromosome 10. The encoded protein has a molar mass of 55.4kDa and spans a length of 499 amino acids. It is expressed in the neurons along with the NF (neurofilament) triplet proteins, NF-L (light), NF-M (medium), NF-H (heavy). |
| Immunogen: | full-length recombinant rat α-internexin. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| WGK Germany | - |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |