Invertase Glycoprotein Standard
SIGMA/I0408 - BioReagent, from Saccharomyces cerevisiae
Synonym: Invertase from baker’s yeast (S. cerevisiae); β-D-Fructofuranosidase; β-D-Fructofuranoside fructohydrolase; Saccharase
CAS Number: 9001-57-4
EC Number: 232-615-7
MDL Number: MFCD00131410
Product Type: Chemical
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| biological source | Saccharomyces cerevisiae |
| concentration | ≥0.5 mg/vial protein (E |
| form | lyophilized powder |
| mol wt | 60 kDa |
| product line | BioReagent |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Application: | Invertase Glycoprotein Standard has been used: • to generate N-linked glycan library • as a negative control to study the binding of lectins to high mannose structures • for sample pre-treatment in proteomic analyses to study drug-induced toxic epidermal necrolysis |
| Application: | The Invertase Glycoprotein Standard can be used to demonstrate N-glycosylation using PNGase F with both in-solution and in-gel procedures. The extent of deglycosylation can be assessed by mobility shift on SDS-PAGE gels. |
| Application: | Used in the production of confectionary foods and artificial honey. |
| Biochem/physiol Actions: | Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products. |
| Other Notes: | Invertase is an enzyme that catalyses the hydrolysis of sucrose into fructose and glucose. Invertase Glycoprotein Standard is the periplasmic (glycosylated form, external invertase) with 50% of its mass as polymannan. Since yeast can provide an alternative system for protein glycosylation that is similar to mammalian systems, periplasmic invertase is often used as a model for the study of the function of oligosaccharides in glycoproteins and for studies on glycoprotein biosynthesis. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.26 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352202 |