Interleukin-2 from mouse
SIGMA/I0523 - IL-2, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: Interleukin-2; T-cell growth factor; mIL-2; mIL-2; IL-2; T-cell growth factor
MDL Number: MFCD00164816
Product Type: Chemical
SDS-PAGE Interleukin-2 from Mouse (Cat. No. I0523) was assessed on SDS-PAGE.
Bioassay EC50 is defined as the effective concentration of Interleukin-2 that elicits a 50% attachment factor in cell based bioassay. Cell line: CTLL2
| assay | ≥97% (SDS-PAGE) |
| biological source | mouse |
| color | white |
| form | lyophilized powder |
| impurities | ≤1.000 EU/μg |
| mol wt | 17.2 kDa |
| packaging | pkg of 20 μg |
| pkg of 5X20 μg | |
| potency | ≤0.2 ng/mL ED50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P04351  |
| Analysis Note: | Activity was determined by dose-dependent stimulation of murine CTLL-2 cells. ED50 : ≤ 0.2 ng/ml (corresponds to a specific activity of 5 × 106 units/mg) |
| Application: | Interleukin-2 from mouse has been used to induce sepsis in rat model. It has also been used in dendritic epidermal T cell culture. |
| Biochem/physiol Actions: | Interleukin-2 (IL-2) is an immunomodulatory factor produced by certain subsets of T lymphocytes. This lymphokine promotes long-term growth of activated T cells and related cell types. IL-2 plays a role in the activation and proliferation of natural killer cells, induces γ-interferon and B cell growth factor secretion, and modulates the expression of the IL-2 receptor. IL-2 expression is known to reduce with age and results in influenza infection, neoplasms and lethal autoimmunity. IL-2 participates in T cell proliferation and differentiation and controls the homeostasis of T cell. As a part of immune response, IL-2 stimulates the action of T helper cell 1 and 2 along with endothelial white blood cells of skeletal muscles. IL-2 regulation is controlled by c-rel protein. IL-2 is a neurotrophic molecule and has special functions in cortical, hippocampal, septal, cerebellar and retinal neurons. |
| General description: | IL-2 (interleukin-2) codes for a pro-inflammatory cytokine. IL-2 is mostly expressed in T helper cells. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in 10 mM sodium citrate, pH 4.0. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |