Anti-Human IgG (whole molecule) antibody produced in goat
SIGMA/I1011 - whole antiserum
Synonym: Anti-Human IgG; Goat Anti-Human IgG
MDL Number: MFCD00162452
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:100 Anti-Human IgG (whole molecule) antibody produced in Goat (Cat. No. I1011) followed by 1:80 Monoclonal Anti-Goat/Sheep IgG -Alkaline Phosphatase antibody produced in Mouse, Clone: GT-34 (Cat. No. A8062).
ELISA Anti-Human IgG (whole molecule) antibody produced in Goat (Cat. No. I1011), followed by 1:5,000 Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A5420), was serially diluted to detect Human IgG by indirect ELISA.
| antibody form | whole antiserum |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:40,000 |
| quantitative precipitin assay: 4.0 mg/mL |
| Application: | Anti-Human IgG (whole molecule) antibody has been used in • enzyme linked immunosorbent assay (ELISA) • printing and processing of reverse phase protein microarrays • western blot • radial immunodiffusion |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant serum immunoglobulin of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. |
| Biochem/physiol Actions: | IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab fragments or bivalent F(ab′)2 fragments. These two enzymes have a broad substrate specificity resulting in heterogenous fragments. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG is present in large quantities in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bon |
| Physical form: | Anti-Human IgG is supplied as a liquid containing 15 mM sodium azide as a preservative |
| Preparation Note: | For continuous use, store at2-8 °C. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |