R3 IGF-1 human
SIGMA/I1146 - recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: Human IGF-1; IGF-1 Protein; Recombinant IGF-1
Product Type: Chemical
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| assay | ≥85% (HPLC) |
| biological source | human |
| form | lyophilized powder |
| mol wt | 7,676 Da |
| packaging | pkg of 0.1 mg |
| potency | ≤10 ng/mL ED50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P05019  |
| Application: | R3 IGF-I is a recombinant analog of insulin-like growth factor containing the complete human IGF-I amino acid sequence with substitution of Arg for Glu3. It was developed as an inexpensive, high quality potent analog of IGF for use as a growth factor supplement for serum-free or low serum cell culture. Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps. |
| Biochem/physiol Actions: | Proliferation of chinese hamster ovary CHO cells. |
| Other Notes: | Recombinant analog of insulin-like growth factor-I with the substitution of Arg for Glu at position 3. |
| Preparation Note: | Prepared from 0.1 M acetic acid |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥85% (HPLC) |
| Storage Temp. | 2-8°C |
| UNSPSC | 51111800 |