Isopropyl β-D-thiogalactopyranoside solution
SIGMA/I1284 - ReadyMade IPTG solution for Blue-white screening
Synonym: IPTG solution
CAS Number: 367-93-1
Empirical Formula (Hill Notation): C9H18O5S
Molecular Weight: 238.30
MDL Number: MFCD00063273
Linear Formula: C9H18O5S
Product Type: Chemical
IPTG induction mechanism illustration. Lac promoters are under the control of Lac repressor and is activated only in the presence of lactose or its synthetic analog IPTG. Like lactose, IPTG binds the repressor and releases his binding to the promoter, but unlike lactose, it′s not metabolized. The release of the repressor allows the transcription of the target gene.
SDS-PAGE Induction of ATF2 expression by IPTG (IPTG Ready Made). The analysis of ATF2 protein induction was performed on 12.5% PhastSystem gel. The induction of
ATF2 is represented by a band at ~50 kDa (red arrow). Assay′s samples were assessed on SDS-PAGE and Stain by eStain L1 Protein Staining System (Cat. No. F2555). Lanes: 1-2: Non IPTG induced cells. 3-4: IPTG induced cells.
ATF2 is represented by a band at ~50 kDa (red arrow). Assay′s samples were assessed on SDS-PAGE and Stain by eStain L1 Protein Staining System (Cat. No. F2555). Lanes: 1-2: Non IPTG induced cells. 3-4: IPTG induced cells.
| description | non-ionic |
| form | liquid |
| grade | Molecular Biology |
| InChI | 1S/C9H18O5S/c1-4(2)15-9-8 |
| InChI key | BPHPUYQFMNQIOC-NXRLNHOXSA |
| mol wt | 238.3 |
| Quality Level | 200  |
| shipped in | dry ice |
| SMILES string | CC(C)S[C@@H]1O[C@H](CO)[C |
| storage temp. | −20°C |
| Application: | Isopropyl β- • used as an inducer for the expression of single domain antibodies(sdAbs) in E coli and bioactive peptides • used in its half-life and decay studies • suitable for induction of recombinant protein expression in systems controlled by the lac repressor |
| Biochem/physiol Actions: | Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β-galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. |
| Biochem/physiol Actions: | Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β--galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. Since Isopropyl β-D-thiogalactopyranoside cannot be metabolized by E. coli its concentration remains constant. Therefore, it is not a variable in the experiment. |
| General description: | Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins in E. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter. IPTG Ready Made is a 0.2 μm filter sterilized solution of 200 mM IPTG. This reagent remains liquid at its storage temperature (-20 °C), thus avoiding freeze-thaw cycles and providing a user-friendly solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |