IMAC-Select Affinity Gel
SIGMA/I1408 - (1:1 suspension in a 30% ethanol solution)
Synonym: Affinity Gel
Product Type: Chemical
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| capacity | 10-30 μmol/mL binding capacity (Cu+2) |
| form | (1:1 suspension in a 30% ethanol solution) |
| impurities | ≤0.08 Metal Contamination (μmol/ml packed gel) |
| matrix | 6% beaded agarose |
| Quality Level | 200  |
| shelf life | 1 yr |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| technique(s) | affinity chromatography: suitable |
| Application: | An immobilized metal affinity chromatography (IMAC) product that can be widely used for purifying proteins, peptides, and nucleotides of interest, most commonly proteins. IMAC-Select utilizes a non-charged linkage chemistry to connect the proprietary quadridentate chelate to the agarose bead matrix. IMAC-Select Affinity Gel is durable and can capture proteins of interest with an affinity to various metal ions such as nickel, cobalt, iron or gallium. A metal free resin allows researchers to add metal of choice, or to use the resin to remove metal contamination from a protein. IMAC-Select Affinity Gel has been used in the affinity purification of kelch-like ECH-associated protein 1 (KEAP1), Wilms tumor gene on the X chromosome (WTX) and glycoprotein G1 tail protein of the andes virus. |
| Other Notes: | Do not allow the affinity gel to remain in any buffer for extended periods of time unless it contains an anti-microbial agent such as 30% ethanol. |
| Physical form: | 1:1 suspension in a 30% ethanol solution |
| Preparation Note: | The ethanol used to store the affiinity gel must be removed prior to use to avoid precipitation of buffer salts.The affinity gel is first washed with 1 to 2 volumes of deionized water to remove ethanol. The gel is charged with 2 to 3 volumes of a solution of the desired metal ion at a concentration of 10 mg/mL, and then equilibrated with 3 to 5 volumes of equilibration buffer. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Risk Statements | 10 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| WGK Germany | WGK 3 |
| Flash Point(F) | 120.2 °F - closed cup |
| Flash Point(C) | 49 °C - closed cup |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |