Pyrophosphatase, Inorganic from baker’s yeast (S. cerevisiae)
SIGMA/I1643 - powder, ≥500 units/mg protein (E1%/280 )
Synonym: Inorganic Pyrophosphatase; Pyrophosphate phosphohydrolase
CAS Number: 9024-82-2
EC Number: 232-784-7
MDL Number: MFCD00131379
Product Type: Chemical
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| form | powder |
| mol wt | 71 kDa |
| Quality Level | 300  |
| specific activity | ≥500 units/mg protein (E |
| storage temp. | −20°C |
| Application: | Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi. It plays an important role in protein, RNA, and DNA synthesis. |
| Application: | Pyrophosphatase has been used: as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA. as a component of PCR mixture |
| Application: | Pyrophosphatase has been used: • as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA. • as a component of PCR mixture. |
| General description: | Molecular Weight: 71 kDa (homodimer consisting of two equal subunits of molecular weight 32-35 kDa) Extinction Coefficient: E1% = 14.5 (280 nm) pI: 4.75 This ubiquitous enzyme serves to drive metabolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if zinc is present. |
| Other Notes: | One unit will liberate 1.0 μmole of inorganic orthophosphate per min at pH 7.2 at 25 °C. |
| Physical form: | Lyophilized powder containing 80-90% buffer salts |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥500 units/mg protein (E |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.6.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |