Anti-IFI-16 antibody, Mouse monoclonal
SIGMA/I1659 - ~1 mg/mL, clone IFI-230, purified from hybridoma cell culture
Synonym: Anti-
MDL Number: MFCD07785406
Product Type: Chemical
Immunoblotting Analysis
Total cell extract of Jurkat cells (Human acute T-cell leukemia) were separated by SDS-PAGE and probed with Monoclonal Anti-IFI-16 (Clone IF-230, Cat. No. I1659) (1.4 μg/mL) followed by HRP-Goat anti-Mouse (Cat. No. A2304).
Total cell extract of Jurkat cells (Human acute T-cell leukemia) were separated by SDS-PAGE and probed with Monoclonal Anti-IFI-16 (Clone IF-230, Cat. No. I1659) (1.4 μg/mL) followed by HRP-Goat anti-Mouse (Cat. No. A2304).
Immunocytochemistry
Nuclear localization of IFI-16; Jurkat cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100 were stained with Monoclonal Anti-IFI-16, clone IF-230 (Cat. No. I1659) at 80 μg/mL followed by Rabbit anti-Mouse IgG-FITC (A). For nuclear localization, the cells were further treated with DAPI (B).
Nuclear localization of IFI-16; Jurkat cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100 were stained with Monoclonal Anti-IFI-16, clone IF-230 (Cat. No. I1659) at 80 μg/mL followed by Rabbit anti-Mouse IgG-FITC (A). For nuclear localization, the cells were further treated with DAPI (B).
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | IFI-230, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 85-95 kDa (cluster of three isotypes A, B, and C) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using whole extract of the Jurkat cell line | |
| UniProt accession no. | Q16666  |
| Biochem/physiol Actions: | The gene IFI-16 (interferon, γ-inducible protein 16) encodes an interferon-inducible transcriptional modulator from the p200-protein family. It may play a role in negative regulation of transcription. Overexpression of this gene in older populations of human fibroblasts leads to cellular senescence. It is also associated with senescence-associated irreversible cell growth arrest in normal human prostate epithelial cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The gene IFI-16 (interferon, γ-inducible protein 16) encodes a member of the interferon-inducible nuclear proteins family containing one or two copies of a conserved 200 amino acid repeat domain called HIN-200 domain. It has three different isoforms, IFI-16A, -16B, -16C. The IFI-16A isoform is the longest with 785 amino acids. IFI-16B is the most broadly expressed isoform with 729 amino acids and IFI-16C has 56 amino acids lesser. The three isoforms have serine and threonine residues which can be phosphorylated. The N-terminus contains an imperfect leucine zipper domain, where the isoforms homo- and heterodimerize. IFI-16 contains an N-terminal domain called DAPIN/PYRIN/CARD that may be involved in protein–protein interactions. IFI-16 is expressed in some haemopoietic lineages , such as CD34+ progenitor cells, mature lymphocytes and monocytes. The gene is mapped to human chromosome 1q22. |
| Immunogen: | synthetic peptide corresponding to amino acids 768-783 at the C-terminus of human IFI-16. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |