Interferon-γ human
SIGMA/I17001 - IFN-gamma, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin tested
Synonym: IFN-γ
MDL Number: MFCD00131391
Product Type: Chemical
Purity
The purity of human, recombinant IFN-γ expressed in HEK 293 cells (Cat. No. I17001) was assessed on SDS-PAGE (1.25 μg/lane) under reducing conditions (+DTT) followed by InstantBlue™ staining (Cat. No. ISB1L).
The purity of human, recombinant IFN-γ expressed in HEK 293 cells (Cat. No. I17001) was assessed on SDS-PAGE (1.25 μg/lane) under reducing conditions (+DTT) followed by InstantBlue™ staining (Cat. No. ISB1L).
Deglycosylation Test
Human, recombinant IFN-γ expressed in HEK 293 cells (Cat. No. I17001) (lanes 1-2) and IFN-γ expressed in E. coli (lanes 3-4) were either treated with N glycosidase F (lanes 1,3) or untreated (lanes 2,4) and separated on SDS-PAGE followed by InstantBlue™ staining (Cat. No. ISB1L).
Lanes
1. HEK 293 I17001 (+ N glycosidase F)
2. HEK 293 I17001 (- N glycosidase F)
3. E. coli (+ N glycosidase F)
4. E. coli (- N glycosidase F)
Human, recombinant IFN-γ expressed in HEK 293 cells (Cat. No. I17001) (lanes 1-2) and IFN-γ expressed in E. coli (lanes 3-4) were either treated with N glycosidase F (lanes 1,3) or untreated (lanes 2,4) and separated on SDS-PAGE followed by InstantBlue™ staining (Cat. No. ISB1L).
Lanes
1. HEK 293 I17001 (+ N glycosidase F)
2. HEK 293 I17001 (- N glycosidase F)
3. E. coli (+ N glycosidase F)
4. E. coli (- N glycosidase F)
Bioassay The biological activity of human, recombinant IFN-γ expressed in HEK293 cells (Cat. No. I17001) versus control IFN-γ from E. coli was measured in a viral resistance assay. The ED50 is defined as the effective concentration of IFN-γ that allows 50% cell growth in an antiviral cell based bioassay. The ED50 of recombinant IFN-γ expressed in HEK293 cells (Cat. No. I17001) is 15 fold more active than the E. coli expressed version. (Representative result)
| assay | ≥98% (SDS-PAGE) |
| form | lyophilized powder |
| mol wt | 16 kDa (glycosylated) |
| potency | ≤0.250 ng/mL In Viral Resistance Assay ED50 |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| storage temp. | −20°C |
| suitability | endotoxin tested |
| technique(s) | cell culture | mammalian: suitable |
| Analysis Note: | The biological activity of recombinant human IFN-γ was tested in culture in a viral resistance assay. The ED50 is defined as the effective concentration of IFN-γ that allows 50% cell growth in an antiviral cell based bioassay. |
| Application: | Interferon-γ human has been used in cytometric bead array to measure the levels of interferon-γ in the sample. |
| Application: | It has also been used for cytokine treatment of human islets to evaluate the activation of hypoxia inducible factor 1 α (HIF1α) targets, in vitro. |
| Biochem/physiol Actions: | IFN-γ is a dimerized soluble cytokine that is the only member of the type II class of interferons. Interferon-γ (IFN-γ) plays an essential role in function of virtually all immune cells and both innate and adaptive immune responses. IFN-γ exhibits various biological effects, such as antiviral activity, inhibition of cell or tumor growth and promotion of terminal differentiation of B cells into immunoglobulin-producing cells. This cytokine also activates macrophages, increases cytotoxicity of natural killer cells and promotes T cell cytotoxicity. In addition to antiviral activity, recombinant human IFN-γ is a potent modulator of immune responses and modifies cellular processes. |
| General description: | Interferon-γ (IFN-γ) is a pleotropic cytokine, encoded by the gene mapped to human chromosome 12q15. It is a non-covalent homodimer with two identical 17kDa polypeptide chains. It belongs to the type II class of interferons. |
| General description: | Recombinant human Interferon-γ (IFN-γ) is expressed in human 293 cells as a glycoprotein with a calculated molecular mass of 16 kDa. This protein is manufactured in human cells using an all-human production system, with full chemically defined ingredients and with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture. The bioactivity of IFN-γ expressed in human 293 cells is significantly higher compared to bacterially expressed IFN-γ. |
| Other Notes: | QDPYVKEAENLKKYFNAGHSDVADN |
| Physical form: | Supplied as a lyophilized powder containing phosphate buffered saline. |
| Hazard statements | H412 |
| Precautionary statements | P273 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |