Interleukin-13 human
SIGMA/I1771 - ≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: IL-13
CAS Number: 148157-34-0
MDL Number: MFCD00212209
Product Type: Chemical
SDS-PAGE Interleukin-13 Human (Cat. No. I1771) was assessed on SDS-PAGE.
Bioassay EC50 is defined as the effective concentration of Interleukin-13 that elicits a 50% attachment factor in cell based bioassay. Cell line: TF-1
| assay | ≥97% (SDS-PAGE) |
| biological source | human |
| color | white |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | 12 kDa |
| packaging | pkg of 10 μg |
| potency | <10.000 ng/mL ED50 |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P35225  |
| Biochem/physiol Actions: | IL-13 is known to be overexpressed in glioblastoma tumors and cell lines. Elevated IL-13 expression is also observed in parasitic infections. It stimulates fibrosis in many infectious diseases, and acts as a profibrogenic factor. |
| Biochem/physiol Actions: | Interleukin-13 is a pleiotropic cytokine produced by activated Th2 cells in the mouse and human. IL-13 is secreted mainly as an unglycosylated protein of 132 amino acids with a molecular weight of 10,000 daltons. A 4.3kb DNA fragment of the mouse IL-13 gene was sequenced and occurs as a single copy mapping a chromosome 11. In the human, a 4.6 kb DNA segment of the IL-13 gene occurs as a single copy and maps to chromosome 5. IL-13 induces B cell proliferation and also induces IgE switching. The proliferative activity of human and mouse IL-13 products are tested in culture using the human erythroleukemic cell line TF-1. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 250 μg BSA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |