Anti-Importin α antibody, Mouse monoclonal
SIGMA/I1784 - clone IM-75, purified from hybridoma cell culture
Synonym: Anti-Imp α; Anti-Qip1; Anti-karyopherin α
MDL Number: MFCD06798706
Product Type: Chemical
Immunoblotting
Cell extract from HeLa cells (Human Cervix Epitheloid Carcinoma) was separated by SDS-PAGE and probed with Monoclonal Anti-Importin α, clone IM-75 (Cat. No. I1784) at different concentrations 2, 1, 0.5, 0.25 μg/mL (Lanes 1-4) or without primary antibody (Lane 5) followed by Goat Anti-Mouse, Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate.
Cell extract from HeLa cells (Human Cervix Epitheloid Carcinoma) was separated by SDS-PAGE and probed with Monoclonal Anti-Importin α, clone IM-75 (Cat. No. I1784) at different concentrations 2, 1, 0.5, 0.25 μg/mL (Lanes 1-4) or without primary antibody (Lane 5) followed by Goat Anti-Mouse, Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | IM-75, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen ~60 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-2 μg/mL using HeLa cell extract | |
| UniProt accession no. | O00629  |
| Application: | Anti-Importin α antibody, Mouse monoclonal has been used: • enzyme linked immunosorbent assay (ELISA) • immunoblotting • immunoprecipitation • immunocytochemistry |
| Application: | Monoclonal Anti-Importin α antibody produced in mouse has been used in western blotting. |
| Biochem/physiol Actions: | The gene KPNA4 (karyopherin subunit α 4) encodes a 16kDa protein, also referred to as importin α 3 belonging to the family of karyopherins (designated KPNA1 to KPNA4). It participates in the recognition of NLS (nuclear localization sequence) sites and dock NLS-containing proteins destined for transport into the nuclear pore complex. It binds to translocon and participates in the sorting of INM (inner nuclear membrane)-directed integral membrane proteins. It associates with nucleoporins and functions in cargo-translocation across the nuclear membrane. Each KPNA contains an N-terminal repeat domain called the importin β binding site that binds to KPNB1, which in turn directs each KPNA/cargo complex through the nuclear pore. The encoded shuttling protein also functions in NF (nuclear factor)-κB nuclear translocation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Importin α (mouse IgG2b isotype) is derived from the hybridoma IM-75 produced by the fusion of mouse myeloma cells (NS1 cells), and splenocytes from BALB/c mice, immunized with recombinant human importin α. The Importin α family of proteins is comprised of three subfamilies based on amino acid sequence similarity: SRP1-like subfamily (containing the SRP1, importin α5, α6 and α7), Rch1-like subfamily (containing Rch1, pendulin, importin α2), and α3/Qip1-like subfamily (containing α4). |
| Immunogen: | recombinant human importin α. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |