Insulin-transferrin-sodium selenite media supplement
SIGMA/I1884 - γ-irradiated, lyophilized powder, BioXtra, suitable for cell culture
Synonym: ITS Supplement
Product Type: Chemical
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| biological source | human plasma |
| form | lyophilized powder |
| product line | BioXtra |
| Quality Level | 200  |
| shipped in | ambient |
| sterility | γ-irradiated |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01308  |
| Application: | Insulin-transferrin-sodiu • medium 199 (M199) media supplement for cardiac myocytes culture • Roswell Park Memorial Institute (RPMI) 1640 medium component for pluripotent stem cell (iPSC) culture • Dulbecco′s Modified Eagle Medium (DMEM) supplement in ID8 ovarian carcinoma cells |
| General description: | Insulin-transferrin-sodiu |
| General description: | Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies. These important factors include insulin, transferrin, selenium, pyruvate, and ethanolamine. INSULIN is a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe observed mitogenic effect to this property. TRANSFERRIN is an iron-transport protein. Iron is an essential trace element, but can be toxic in the free form. To nourish cells in culture, it is supplied bound to transferrin in serum. SELENIUM is an essential trace element normally provided by Serum. Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum. |
| Other Notes: | Each vial contains: ≥21 mg recombinant human insulin; ≥19 mg human transferrin (substantially iron free), and 25 μg sodium selenite. |
| Other Notes: | ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate. |
| Preparation Note: | Each vial sufficient to prepare 5 liters of medium. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352205 |