Anti-Human IgG (whole molecule) antibody produced in rabbit
SIGMA/I2011 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-IgG antibody; Rabbit anti-Human IgG
MDL Number: MFCD00162452
Product Type: Chemical
ELISA Anti-Human IgG (whole molecule) antibody produced in Rabbit (Cat. No. I2011), followed by 1:5,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545), was serially diluted to detect Human IgG by indirect ELISA.
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 300  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:80,000 |
| quantitative precipitin assay: 3.0-4.0 mg/mL |
| Application: | Anti-Human IgG (whole molecule) antibody produced in rabbit has been used in immunoprecipitation and enzyme linked immuno sorbent assay (ELISA). |
| Application: | Anti-Human IgG (whole molecule) antibody produced in rabbit was coated on Sepharose beads to immunoprecipitate IgG from HUVEC cultured conditioned medium. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. |
| Biochem/physiol Actions: | The antiserum is determined to be immunospecific for human IgG. Reactivity with light chains is observed. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulin have two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein, usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 % of the total immunoglobulin consists of IgG. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative |
| Preparation Note: | For continuous use, store at 2-8 °C. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is notrecommended.If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |