Anti-Human IgG (Fc specific) antibody produced in goat
SIGMA/I2136 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti Human IgG Fc - Anti-Human IgG (Fc specific) antibody produced in goat; Anti Human Igg; Anti Human Igg Antibody; Anti-Human Igg; Anti-Human Igg Fc; Goat Anti Human Igg
MDL Number: MFCD00162436
Product Type: Chemical
ELISA 1:10,000 Anti-Human IgG (Fc specific) antibody produced in Goat (Cat. No. I2136) followed by 1:5,000 Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A5420) was probed to detect Human IgG and Human FC by indirect ELISA. Reactivity is observed only with Human IgG and HFc, but not with HFab, HuIgA, HuIgM, H(k+l), MIgG and RatIgG.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 300  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:10,000 |
| Application: | Anti-Human IgG (Fc specific) antibody produced in goat has been used: • in double-capture ELISA to measure antiglobulin responses in serum of transplant patients treated with CD52 monoclonal antibodies (CAMPATH-1G) • in detection of IgG levels rheumatoid arthritis patients • in small intestine biopsies • in patients with acute myocardial infarction by immunoblotting |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Enzyme-linked immunosorbent assay (1 paper) |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. Mutations in the Fc region of IgG is implicated in autoimmune diseases like Rheumatoid arthritis. Engineered Fc proteins have therapeutic significance for the treatment of autoimmune diseases. |
| Biochem/physiol Actions: | Specificity for the Fc fragment of human IgG is determined by ELISA and immunoelectrophoresis (IEP). The antibody preparation is specific for human IgG, Fc fragment when tested against purified human IgA, IgG (Fc and Fab fragments), IgM, Bence Jones kappa, and Bence Jones lambda myeloma proteins. No reactivity is observed with the Fab fragment of human IgG, light chains, IgA, or IgM. The affinity purified anti-human IgG (Fc specific) reagent offers the advantage of increased sensitivity for human IgG without cross reactivity with other substances present on membrane or cell surface. The lack of interspecies cross reactivity with mouse or rat serum proteins makes this product excellent for the screening of human monoclonal antibodies produced by hybridoma cells grown in vivo in mouse or rat ascites fluids. This product has the ability to detect all human IgG subclasses in human biological fluids or tissues of normal or pathological situations such as cancer or autoimmune diseases. It is effective as a second antibody reagent in immunoassay procedures and can be used as starting material for conjugates using enzymes or fluorescent dyes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The product binds human IgG and does not bind other human Igs. Immunoglobulins comprise of two fragment antigen binding (Fab) and one fragment crystallizable (Fc) domains. The gene encoding IgG gene cluster is located on human chromosome 14. Anti-Human IgG (Fc specific) antiserum is produced in goat using purified human IgG, Fc fragment, as the immunogen. Affinity isolated antibody is obtained from goat anti-human IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fc fragment of human IgG. |
| Immunogen: | Anti-Human IgG (Fc specific) antiserum is produced in goat using purified human IgG, Fc fragment, as the immunogen |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |