Anti-Interleukin-6 antibody produced in rabbit
SIGMA/I2143 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-IL-6
MDL Number: MFCD00162579
Product Type: Chemical
Neutralizing ELISA The minimum titer of 1 μg/mL was determined by neutralization bioassay using recombinant Human Interleukin-6. Used at the recommended concentration, the antibody will neutralize the biological activity of 1 unit of IL-6 on T1165 cells.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | neutralization: suitable using human IL-6 in a T1165 cell proliferation bioassay |
| radioimmunoassay: suitable | |
| UniProt accession no. | P05231  |
| Application: | Anti-Interleukin-6 antibody can be used in western blotting and ELISA for studying human IL-6. It can also be used for dot blot immunoassay. Anti-Interleukin-6 antibody can be used for selective neutralization of human IL-6 bioactivity in cell culture. It can also be used in radioimmunoassay (RIA) (diluted 1:60,000). |
| Application: | It may also be used in immunoblotting. |
| Biochem/physiol Actions: | Interleukin 6 (IL-6) acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells, and hepatocytes. Interleukin 6 (IL-6) also potentiates the proliferative effect of Interleukin 3 (IL-3) on multipotential hematopoietic progenitors. |
| Biochem/physiol Actions: | Interleukin-6 (IL-6) is a cytokine produced by activated T cells, endothelial cells, fibroblasts and activated monocytes or macrophages. It plays a crucial role in regulating the acute phase response as well as responses occurs after injury and infection. It interacts with IL-2 and facilitates in the proliferation of T lymphocytes. |
| Biochem/physiol Actions: | The antibody will neutralize the biological activity of human IL-6 in cell culture. The antibody does not cross-react with human IL-1β, TNF-α or mouse IL-6. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Interleukin 6 (IL-6) is produced in rabbit using purified human recombinant IL-6 produced in E. coli as the immunogen. Interleukin-6 is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. Interleukin-6 (IL-6) is a cytokine produced by activated T cells, endothelial cells, fibroblasts and activated monocytes or macrophages. |
| Immunogen: | Purified human recombinant IL-6 produced in E. coli. |
| Physical form: | Solution, 0.2 μm filtered, in 0.5 mL phosphate buffered saline, pH 7.4. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |