Anti-Importin β antibody,Mouse monoclonal
SIGMA/I2534 - clone 31H4, purified from hybridoma cell culture
Synonym: Anti-Imp β; Anti-karyopherin β1; Anti-p97
MDL Number: MFCD07785407
Product Type: Chemical
Immunoblotting
Cell extracts from HeLa cells (Human Cervix Epitheloid Carcinoma) were separated by SDS-PAGE, blotted with (Lane 1) or without (Lane 2) Monoclonal Anti-Importin β, clone 31H4 (Cat. No. I2534) and developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Cell extracts from HeLa cells (Human Cervix Epitheloid Carcinoma) were separated by SDS-PAGE, blotted with (Lane 1) or without (Lane 2) Monoclonal Anti-Importin β, clone 31H4 (Cat. No. I2534) and developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 31H4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~97 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, mouse, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| western blot: 0.5-1 μg/mL using HeLa total cell extract | |
| UniProt accession no. | Q14974  |
| Application: | Anti-Importin β antibody,Mouse monoclonal has been used in: • enzyme linked immunosorbent assay (ELISA) • immunocytochemistry • immunofluorescent staining • coimmunoprecipitaion • western blotting • immunofluorescence • immunoprecipitation |
| Biochem/physiol Actions: | Importin β (Impβ) forms a complex with importin α, which, in turn, binds the cargo protein via its NLS (nuclear localization signals). The cargo protein is transported by this ternary complex to the cytoplasmic face of the nuclear pore complex (NPC) via a direct interaction with specific nucleoporins. When the complex reaches the nuclear site of the nuclear pore complex (NPC), Ran-GTP binds to Impβ to form an Impβ/Ran-GTP complex and releases Impα and the cargo protein. The Impβ/Ran-GTP complex is then transported back to the cytoplasm, where the complex dissociates upon hydrolysis of GTP to GDP. Importin β is then ready to participate in new nuclear import cycle. |
| Biochem/physiol Actions: | Importin β regulates cellular functions, such as mitotic spindle assembly and centrosome dynamics. It is implicated in the insertion of integral membrane proteins into the inner nuclear membrane. Importin β also functions as a chaperone for highly basic proteins in the cytoplasm. |
| Biochem/physiol Actions: | Monoclonal Anti-Importin β recognizes human, bovine, rat, and mouse importin β. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Importin β comprises 19 tandemly repeated Huntingtin, elongation factor 3 (EF3), protein phosphatase 2A (PP2A), and the yeast kinase serine/threonine-protein kinase-TOR1 (HEAT) motifs and wraps around the importin-β (IBB). It also consists of an N-terminal RAN guanosine triphosphate (GTP) binding domain, a middle zone interacting with the nuclear pore, and an importin α binding domain at the C-terminus. The importin β gene is mapped to human chromosome location 17q21.32. |
| General description: | Monoclonal Anti-Importin b (mouse IgG1 isotype) is derived from the 31H4 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with importin. Importin β (Imp β), also referred to as karyopherin β1, is an import receptor that belongs to the family of nuclear transport receptors. |
| Immunogen: | importin β |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |