Anti-phospho-Insulin Receptor Substrate-1 (pTyr612) antibody produced in rabbit
SIGMA/I2658 - affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-phospho-IRS-1 (pTyr612)
MDL Number: MFCD05865378
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| packaging | vial of 100 μL |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pTyr612) |
| technique(s) | western blot: suitable |
| UniProt accession no. | P35568  |
| Application: | Anti-phospho-IRS-1 (pTyr612) antibody may be used for detection by immunoblotting. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Assay Dependent. It is recommended that the user determine the appropriate working dilution for their process. |
| General description: | Insulin receptor substrate-1 (IRS-1) is among the four IRS proteins (IRS-1-IRS-4) that mediate signalling by insulin receptor (IR) and insulin-like growth factor (IGF) receptor. IRS-1 has a pleckstrin homology domain and a phosphotyrosine-binding domain. . Phosphorylation of Tyr612 and Tyr632 fully activate the PI3K signalling pathway in response to insulin and induce translocation of GLUT4. Thus IRS-1 protein plays a vital role in mediating the metabolic actions of insulin via the PI3K pathway. Additionally, IRS-1 has the abililty to translocate to the nucleus, the mechanism of which is not very clear. The transcriptional activity of IRS-1 is involved in the development of breast and prostate cancers The antibody recognizes human IRS-1 (pTyr612). It may cross react with in mouse, rat and chicken (100% homologous) IRS-1 (pTyr612). |
| Immunogen: | synthetic phosphopeptide derived from the region of insulin receptor substrate-1 (IRS-1) phosphorylated at tyrosine 612. |
| Physical form: | Solution in Dulbecco′s PBS, pH 7.3, 50% glycerol containing 1 mg/ml BSA and ≤ 0.1% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |