ITS+3 Liquid Media Supplement (100×)
SIGMA/I2771 - liquid, sterile-filtered, BioReagent, suitable for cell culture
Synonym: ITS Supplement
Product Type: Chemical
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| form | liquid |
| impurities | endotoxin, tested |
| product line | BioReagent |
| Quality Level | 300  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | ITS+3 Liquid Media Supplement (100×) has been used as a media supplement:• to support the growth of human adipose stem cells (ASC) • to culture porcine intervertebral disc (IVD) cells and tissues • to culture cartilage endplate (CEP) cells |
| Biochem/physiol Actions: | • Insulin: a polypeptide hormone that promotes glucose and amino acid uptake and may produce a mitogenic effect. • Transferrin: an iron-transport protein. Iron is an essential trace element but can be toxic in its free form. To nourish cells in culture, iron is supplied bound to transferrin in serum. • Selenium: an essential trace element present in serum. |
| General description: | ITS+3 liquid media supplement is a mixture of insulin, human transferrin (partially iron-saturated), sodium selenite, linoleic acid, and bovine serum albumin (BSA). It is suitable for serum-free cell culture applications. |
| Other Notes: | Contains 1.0 mg/ml bovine insulin, 0.55 mg/ml human transferrin (substantially iron-free), 0.5 μg/ml sodium selenite, 470 μg/ml linoleic acid, 470 μg/ml oleic acid and 50 mg/ml bovine serum albumin at the 100x concentration. Contains 2 moles each of linoleic acid and oleic acid per mole of albumin. |
| Preparation Note: | Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352205 |