StableCell™ IMDM
SIGMA/I2911 - With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Synonym: Iscove’s Modified Dulbecco’s Medium; IMDM
MDL Number: MFCD00217469
Product Type: Chemical
| components | NaHCO3: yes glucose: yes stable glutamine: yes phenol red: yes |
| form | liquid |
| impurities | Endotoxin, tested |
| Quality Level | 400  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | hybridoma: suitable |
| cell culture | mammalian: suitable |
| Application: | Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells. |
| General description: | This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures. |
| Legal Information: | StableCell is a trademark of Sigma-Aldrich Co. LLC |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |