Interleukin-10 from mouse
SIGMA/I3019 - >97% (SDS-PAGE), recombinant, expressed in E. coli, suitable for cell culture
Synonym: IL-10
MDL Number: MFCD00212207
Product Type: Chemical
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| assay | >97% (SDS-PAGE) |
| biological source | mouse |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | ~18 kDa |
| packaging | pkg of 5 μg |
| potency | 0.01-1.50 ng/mL EC50 |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P18893  |
| Analysis Note: | The biological activity of IL-10 is determined in a cell proliferation assay using MC/9 cells. |
| Application: | Interleukin-10 from mouse has been used as a standard in ELISA (enzyme-linked immunosorbent assay) cytokine assay. |
| Biochem/physiol Actions: | Interleukin-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1+ (CD5+), and normal B cells. In human, the cellular sources of IL-10 consist of CD4+ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors. |
| Biochem/physiol Actions: | Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors. |
| Biochem/physiol Actions: | Regulates lymphoid and myeloid cell functions; blocks the activation of cytokine synthesis and several accessory functions of macrophages; enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |