ITS Liquid Media Supplement (100×)
SIGMA/I3146 - liquid, sterile-filtered, BioReagent, suitable for cell culture
Synonym: Human ITS Liquid Media; Insulin-
Product Type: Chemical
| form | liquid |
| impurities | endotoxin, tested |
| product line | BioReagent |
| Quality Level | 500  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| single cell analysis: suitable |
| Application: | ITS Liquid Media Supplement (100×) has been used as a media supplement to culture: • α mouse liver 12 (AML-12) cells • rat fetal testis explants • human dental pulp stem cells (DPSC) |
| Biochem/physiol Actions: | • Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property. • Transferrin: an iron-transport protein. • Selenium: an essential trace element normally provided by serum. |
| Other Notes: | Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration. |
| Other Notes: | ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate. |
| Preparation Note: | Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352205 |