Interferon-γ human
SIGMA/I3265 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE), lyophilized powder, suitable for cell culture
Synonym: IFN-γ
MDL Number: MFCD00131391
Product Type: Chemical
SDS-PAGE The purity of recombinant Human INF-γ (Cat. No. I3265) was determined by SDS-PAGE of reduced INF-γ and staining with Coomassie Blue.
| assay | ≥98% (SDS-PAGE) |
| biological source | human |
| color | white |
| form | lyophilized powder |
| impurities | ≤1 EU/μg endotoxin |
| mol wt | ~17 kDa |
| packaging | pkg of 0.1 mg |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| solubility | water: soluble 1.000 mL, clear, colorless |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01579  |
| Analysis Note: | The biological activity is measured by a viral resistance assay. |
| Application: | Interferon-γ human has been used: • to study the effect of interferon-γ on cultured trophoblasts in absence and presence of fibronectin • to analyse factors which participate in the production of antibody microarrays on glass support • for ELISA (enzyme-linked immunosorbent assay) method to understand the effect of interferon-γ in human gingival fibroblasts in presence of bacterial challenge |
| Biochem/physiol Actions: | IFNG (interferon-γ) is a type-2 interferon which is produced by Th (T helper cells)-1 lymphocytes, CD8 cytotoxic lymphocytes, natural killer (NK) cells, and natural killer T (NKT) cells. It plays an important role in innate and adaptive immune responses, and helps in defense against bacterial infections, dimorphic yeast and some viral infections. High expression of IFNG is common in many autoimmune diseases, such as systemic lupus erythematosis, Sjögren syndrome, inflammatory bowel disease and multiple sclerosis. |
| Physical form: | Lyophilized from phosphate buffered saline. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |