Anti-Human IgG (Fc specific), F(ab′)2 fragment antibody produced in goat
SIGMA/I3391 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat anti-human IgG
MDL Number: MFCD00162436
Product Type: Chemical
ELISA 1:20,000 Anti-Human IgG (Fc specific), F(ab′)2 fragment antibody produced in Goat (Cat. No. I3391), followed by 1:10,000 Anti-Goat IgG (whole molecule)-Alkaline Phosphatase antibody produced in Rabbit (Cat. No. A4187), was diluted to detect Human IgG (Fc specific) by indirect ELISA. Reactivity is observed with Human IgG and Human Fc; but not with Human Fab, Human Kappa+Lambda, Human IgA, Human IgM, Mouse IgG, and Rat IgG.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| concentration | 2.0 mg/mL protein |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Application: | Anti-Human IgG (Fc specific), F(ab′)2 fragment antibody produced in goat has been used in studying the antibody binding assay of soluble intercellular adhesion molecule-1 (ICAM-1)-Fc. |
| Application: | Anti-human IgG (Fc specific), F(ab’)2 fragment antibody can be used in ELISA to determine human IgG1-Fc protein. |
| Biochem/physiol Actions: | IgG stimulates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a crucial role in antibody-dependent cell-mediated cytotoxicity (ADCC). IgG might be associated with allergy. It has the longest serum half life. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG antibody plays a crucial role in humoral immune responses such as phagocytosis, complement activation, placental transport and cell surface-receptor binding. Anti-human IgG (Fc specific), F(ab’)2 fragment antibody can be used as a capture antibody in antibody-binding assay for soluble ICAM-1-Fc. Goat anti-human-IgG (Fc specific) F(ab’)2 antibody reacts specifically with human IgG but does not react with other immunoglobulins. |
| General description: | IgG is the main type of antibody in human serum, secreted by the B cells. It consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are linked by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4. |
| Immunogen: | Purified human IgG |
| Other Notes: | Antibody adsorbed with mouse and rat IgG |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% sodium azide |
| Preparation Note: | Adsorbed to reduce background with mouse or rat samples. Useful when trying to avoid background staining due to the presence of Fc receptors. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |