Insulin-like Growth Factor-I human
SIGMA/I3769 - IGF-I, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: IGF; IGF-I; somatomedin c
MDL Number: MFCD00081716
Product Type: Chemical
Bioassay Basic growth factor is a protein that is mitogenic for a variety of cell types. IGF-I potency is quantified by its ability to stimulate a growth of Balb/3T3 cells in serum free conditions. The relative cell growth is detected using a fluorimetric assay based on the measurement of the conversion of Resazurin to Resorufin by metabolically active cells. The amount of IGF-I that will give 50% of the maximal proliferation (EC50) is calculated from the different amounts assayed.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: I3769-50UG
| assay | ≥95% (HPLC) |
| biological source | human |
| form | lyophilized powder |
| impurities | ≤1.00 EU/μg |
| mol wt | ~7.6 kDa |
| packaging | pkg of 50 and 100 μg |
| potency | 0.1-10 ng/mL ED50/EC50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P05019  |
| Analysis Note: | The biological activiy is calculated by the dose-dependent proliferation of murine BALBC/3T3 cells. ED50 corresponds to a specific activity of at least 1 x 105 units/mg. |
| Application: | Insulin-like Growth Factor-I human has been used: • in modified Dulbecco minimal essential medium for in vitro culture of testicular cells • in culture media, to study its effect on steroidogenesis during testicular development • in culture media for in vitro assay of splenic pro-erythroid cell growth and differentiation |
| Biochem/physiol Actions: | IGF-I also known as somatomedin C, is secreted from the liver into circulation in a process regulated by pituitary growth hormone (GH) and so it mediates the growth-promoting activity of GH. In the developing embryo IGF-I is expressed primarily by mesenchymal-derived cells. After birth IGF-I expression in most extrahepatic tissues declines and hepatic expression of IGF-I becomes GH-regulated. Expression of IGF-I outside the liver is regulated differently, depending on the specific tissues. For example, gonadotropins and sex steroids regulate IGF-I expression within the reproductive system, while parathyroid hormone and sex steroids regulate IGF-I expression in bone. IGF-I is produced in several human tumors. IGF-I is mitogenic for a variety of cells including fibroblasts, osteoblasts, smooth muscle cells, fetal brain cells, neuroglial cells, and erythroid progenitor cells. IGF-I exerts its actions exclusively through the IGF-I receptor (IGF-IR). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis. |
| Biochem/physiol Actions: | IGF-I, also known as somatomedin C, mediates the growth-promoting activity of GH (growth hormone). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis. IGF-I exerts its actions through the IGF-I receptor. |
| Biochem/physiol Actions: | Insulin-like growth factor I (IGF-I) plays a critical role in cellular energy metabolism and growth and development, especially prenatal growth. |
| General description: | Insulin-like growth factor I (IGF-I) is localized on human chromosome 12q23.2. |
| Physical form: | Lyophilized from 0.2 μm filtered solution in 50 mM acetic acid. |
| Preparation Note: | Reconstitute in water |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |