Protease Inhibitor Cocktail, Animal Component Free
SIGMA/I3786 - for use with mammalian cell and tissue extract, DMSO solution
Synonym: Protease inhibitor
Product Type: Chemical
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| form | DMSO solution |
| Quality Level | 200  |
| solubility | water: soluble |
| storage temp. | −20°C |
| Application: | Animal component free (ACF). Optimized and tested for general use. Can also be used for purification of histidine-tagged proteins, as chelating agents have been omitted. |
| Biochem/physiol Actions: | Inhibits serine, cysteine, aspartic proteases, and aminopeptidases. |
| General description: | Components of this cocktail are of non-animal origin and no animal products were used in the component production process. All vessels and instruments used for the cocktail production are dedicated for animal component free production and have never encountered an animal product. Aprotinin used in this cocktail is a recombinant bovine protein expressed in plants (Nicotiana). |
| General description: | This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts. |
| Other Notes: | AEBSF Aprotinin Bestatin E-64 Leupeptin Pepstatin A |
| Physical form: | The components in this ACF protease inhibitor cocktail do not originate from animal sources and can thus be used in the production of animal-free proteins. |
| Physical form: | This ACF cocktail is supplied as a ready-to-use solution in DMSO. One ml of the cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g (wet weight) of bovine liver, or 10 mL of cell lysate obtained from CHO cells at a cell density of 108 cells per mL. |
| RIDADR | NA 1993 / PGIII |
| WGK Germany | WGK 2 |
| Flash Point(F) | 188.6 °F - closed cup |
| Flash Point(C) | 87 °C - closed cup |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |