Interleukin-3 from mouse
SIGMA/I4144 - IL-3, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: IL-3; mIL-3; Interleukin-3 from mouse
MDL Number: MFCD00164817
Product Type: Chemical
SDS-PAGE Interleukin-3 from Mouse (Cat. No. I4144) was assessed on SDS-PAGE.
Bioassay EC50 is defined as the effective concentration of Interleukin-3 that elicits a 50% attachment factor in cell based bioassay. Cell line: M-NFS-60
| assay | ≥98% (SDS-PAGE) |
| biological source | mouse |
| color | white to yellow |
| form | lyophilized powder |
| impurities | ≤1.000 EU/μg |
| mol wt | 15.1 kDa |
| packaging | pkg of 10 and 50 μg |
| potency | 0.01-0.1 ng/mL ED50/EC50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01586  |
| Analysis Note: | The biological activity is measured by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells. |
| Application: | Interleukin-3 from mouse has been used for the determination of 5-lipoxygenase product leukotriene C4 (LTC4) formation. It has also been used as a component of RPMI 1640 medium for cell transfection. |
| Biochem/physiol Actions: | Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor. |
| General description: | Interleukin-3 is a hematopoietic growth factor, which stimulates basophil development and triggers histamine, IL-4 and IL-6 release. It improves monocyte and macrophage activation and phagocytosis, enhances the secretory function of eosinophils. Interleukin-3 promotes endothelial cell proliferation and migration. |
| Physical form: | Lyophilized with 0.5 mg of bovine serum albumin. Sterile filtered through a 0.2 μm filter. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |