Interleukin-4 human
SIGMA/I4269 - IL-4, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: IL-4; Interleukin-4 human; hIL-4; hIL-4
MDL Number: MFCD00151705
Product Type: Chemical
SDS-PAGE Interleukin-4 Human (Cat. No. I4269) was assessed on SDS-PAGE.
Bioassay EC50 is defined as the effective concentration of Interleukin-4 that elicits a 50% attachment factor in cell based bioassay.
| assay | ≥98% (SDS-PAGE) |
| biological source | human |
| color | white |
| form | lyophilized powder |
| impurities | ≤1.000 EU/μg |
| mol wt | 14.9 kDa (containing 129 amino acid residues) |
| packaging | pkg of 5 and 50 μg |
| potency | ≤0.400 ng/mL EC50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P05112  |
| Analysis Note: | The biological activity is measured by the dose-dependent stimulation of the proliferation of human TF-1 cells. |
| Application: | Lymphokine with profound effects on the growth and differentiation of immunologically competent cells, inhibits VEGF-induced and βFGF-induced angiogenesis. |
| General description: | Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including differentiation of naive T cells into the TH2 phenotype, promoting B cell proliferation, antibody isotype switching, and expression of other TH2 cytokines including IL-5 and IL-9.1,2 IL-4 plays a critical role in the development of allergic inflammation and asthma.3 IL-4 binds to two distinct receptors; the type I receptor, a heterodimer consisting of the IL-4Rα chain and the common gamma chain, γc, and the type II receptor, a heterodimer of IL-4Rα and IL-13Rα1.4 Naturally occurring IL-4 has a molecular mass of 12-20 kDa. Recombinant human IL-4 is a 14.9 kDa protein containing 129 amino acid residues. Human and mouse IL-4 share a 50% amino acid sequence homology, but their biological actions are species-specific.5 |
| Physical form: | Lyophilized from a sterile filtered solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |