Invertase from Candida utilis
SIGMA/I4753 - Grade X, ≥300 units/mg solid
Synonym: β-D-Fructofuranoside fructohydrolase; β-Fructofuranosidase; Saccharase
CAS Number: 9001-57-4
EC Number: 232-615-7
MDL Number: MFCD00131410
Product Type: Chemical
Figure 1. Stability (powder form); kept under dry conditions
Figure 2. pH Activity; 20 °C, 3-min. reaction with the buffer soution: pH 2–3, 0.1 M glycine-HCl; pH 4–5, 50 mM acetate; pH 6~7, 50 mM phosphate
Figure 3. Temperature activity; 3-min. reaction in 50 mM acetate buffer, pH 4.5
Figure 4. pH Stability; 50 °C, 10-min. treatment with the buffer solution: pH 2~3, 0.1 M glycine-HCl; pH 4~5, 50 mM acetate; pH 6~8, 50 mM phosphate
Figure 5. Thermal stability; 10-min. treatment with 50 mM acetate buffer, pH 4.5
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: I4753-1G
| foreign activity | α-galactosidase (melibiase), essentially free |
| form | solid |
| mol wt | ~260 kDa |
| Quality Level | 200  |
| specific activity | ≥300 units/mg solid |
| storage temp. | −20°C |
| type | Grade X |
| Application: | Invertase from Candida utilis has been used: • as a supplement in YE liquid medium to observe for morphological changes in Streptomyces sp. • to determine the sucrose content in ethanolic extract of leaf tissues • to determine the total non-structural carbohydrates (TNC) in root tissues |
| Application: | This enzyme is useful for enzymatic determination of saccharose and for the structure investigation of carbohydrates containing β-D-fructofuranoside residue. |
| Biochem/physiol Actions: | Invertase acts on sucrose and cleaves it into glucose and fructose. It is used in the preparation of invert syrup with equimolar concentration of fructose and sucrose. It finds its applications in confectionaries and in the production of alcoholic beverages, glycerol and lactic acid. Invertase is also used in the preparation of artificial honey, plasticizers for cosmetic industries. |
| General description: | Invertase from Candida utilis is secreted in the periplasm. The molecular weight of invertase from C. utilis is 300 kDa. It is a dimer of identical subunits. It is a N-glycosylated protein, rich in mannose oligosaccharide in its structure. The invertase encoding gene of C. utilis shares high homology with SUC2 gene of Saccharomyces cerevisiae. |
| General description: | Michaelis constant : 1.5x10 -2M (Saccharose) Structure : Glycoprotein containing ca. 50% of carbohydrates Optimum pH : 3.5 - 4.0 Optimum temperature : 60 - 70oC pH Stability : pH 4.0 - 6.0 (50oC, 10min) Thermal stability : below 60oC (pH 4.5, 10min) Substrate specificity : The enzyme hydrolyzes saccharose and raffinose, but does not hydrolyze inulin and melezitose.6) |
| Other Notes: | One unit will hydrolyze 1.0 μmole of sucrose to invert sugar per min at pH 4.5 at 55°C. |
| Packaging: | 1 g in poly bottle |
| Preparation Note: | Chromatographically purified |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| activity | specific activity: ≥300 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.26 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |