Isocitrate Dehydrogenase 1 (NADP+) human
SIGMA/I5036 - recombinant, expressed in E. coli, lyophilized powder, ≥80 units/mg protein
Synonym: IDH1; Isocitrate Dehydrogenase Cytoplasmic
CAS Number: 9028-48-2
Product Type: Chemical
SDS-PAGE Isocitrate Dehydrogenase 1 (NADP+) Human (Cat. No. I5036) was assessed on SDS-PAGE.
| assay | ≥90% (SDS-PAGE) |
| form | lyophilized powder |
| mol wt | 46,658 Da |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| specific activity | ≥80 units/mg protein |
| storage temp. | −20°C |
| Biochem/physiol Actions: | Isocitrate dehydrogenase 1 (IDH1) help in the oxidative carboxylation of isocitrate to α-ketoglutarate, that results in the formation of reduced nicotinamide adenine dinucleotide phosphate (NADPH). It participates in lipid metabolism and glucose sensing. Suppressing IDH1 in pancreatic islets affects the insulin secretion, induced by glucose and leads to increase in lactate production. |
| General description: | Isocitrate dehydrogenase 1 (IDH1) gene is located on human chromosome 2q33. IDH1 is present in the cytoplasm and peroxisomes. It is expressed at high levels in the mammalian liver. IDH1 consists of a C-terminal tripeptide peroxisome, that targets the signal one sequence. |
| Other Notes: | One unit corresponds to the amount of enzyme which converts 1.0 μmol DL-isocitrate to α-ketoglutarate per minute at pH 7.4 and 37 °C (NADP as cofactor). |
| Physical form: | Supplied as a lyophilized powder containing Tris-HCl pH 8.0, trehalose, ammonium sulfate and DTT |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ≥80 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1.1.42 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |