IgG1, Kappa from human myeloma plasma
SIGMA/I5154 - purified immunoglobulin, >95% (SDS-PAGE)
Synonym: Human IgG1-κ
MDL Number: MFCD00163924
Product Type: Chemical
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| antibody form | purified immunoglobulin |
| application(s) | research pathology |
| assay | >95% (SDS-PAGE) |
| biological source | human |
| conjugate | unconjugated |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Analysis Note: | Identity is determined by immunoelectrophoresis and indirect ELISA. |
| Application: | The purified IgG1, κ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Human myeloma IgG1, κ was injected into mice (500 μg) to assess the T cell and B cell responses and also used for flow cytometry in CHO cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG1 is the most abundant of the IgG subclass Human myeloma IgG1, κ is purified from human plasma by fractionation, ion-exchange, and affinity chromatography procedures. |
| Physical form: | Solution in 0.02 M Tris buffered saline, pH 8.0. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |