Interleukin-7 human
SIGMA/I5896 - ≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: IL-7
MDL Number: MFCD00163948
Product Type: Chemical
Bioassay The procedure measures the amount of IL-7 β that will cause 50% of the maximal proliferation of 2E8 cell line. These cells need IL-7 β for their growth and will not grow in the absence of this cytokine. Induce proliferation by adding exogenous IL-7 β. The growth of the cells, after 48 hours, is measured using the Resazurin staining.
| assay | ≥98% (SDS-PAGE) |
| biological source | human |
| color | white |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | 25 kDa |
| packaging | pkg of 5 μg |
| potency | ≤0.500 ng/mL EC50 |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| solubility | water: soluble 0.005-0.010 mL |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P13232  |
| Biochem/physiol Actions: | IL-7 helix A is crucial for its interaction with the interleukin-7 human receptor (IL-7Rα). It is reported to enhance immunity during viral infections and may be useful in cancer immunotherapy. Mutation in the IL-7 gene is implicated in CD4+ T-cell lymphopenia. |
| Biochem/physiol Actions: | Interleukin-7 is a lymphoid cell growth factor which affects pre-B, pro-B and early T cells. It supports the growth of early B cells and is mitogenic for thymocytes and co-mitogenic with PHA and Con A. IL-7 stimulates the proliferation of CD4/CD8 cells. Human and mouse IL-7 have 60% amino acid sequence identity. |
| Biochem/physiol Actions: | Interleukin-7 is a lymphoid cell growth factor which affects pre-B, pro-B and early T cells. It was first isolated by Namen in 1988. IL-7 has a number of known activities. It supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic for thymocytes and co-mitogenic with PHA and Con A. IL-7 also stimulates the proliferation of CD4/CD8 cells. The proliferative response of thymocytes to IL-7 is not affected by antibodies to the T cell growth factors such as IL-2, IL-4, and IL-6. Mature T cells respond to IL-7 and Con A, but not to IL-7 alone. Antibodies against IL-2 affect its activity, suggesting that it functions through IL-2 production. Human natural IL-7 is 25 kDa glycoprotein that has 6 cysteine residues which are important for biological activity. Human and mouse IL-7 have 60% amino acid sequence homology. |
| Biochem/physiol Actions: | Lymphoid cell growth factor which affects pre-B, pro-B and early T cells; supports the growth of early B cells and is mitogenic for thymocytes and co-mitogenic with PHA and Con A; stimulates the proliferation of CD4/CD8 cells. |
| General description: | The interleukin-7 human (IL-7) gene is mapped to human chromosome 8q21.13. It is a non-hematopoietic cell-derived cytokine. IL-7 is majorly produced by the fibroblastic stromal cells, dendritic cells, thymus epithelial cells and skin keratinocytes. Structurally, IL-7 takes up an up-up-down-down 4-helix bundle topology and has two crossover loops. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in 10 mM acetic acid containing 250 μg bovine serum albumin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |