Pyrophosphatase, Inorganic from Escherichia coli
SIGMA/I5907 - recombinant, expressed in E. coli, lyophilized powder, ≥90%, ≥800 units/mg protein
Synonym: Inorganic Pyrophosphatase; Pyrophosphate phosphohydrolase
CAS Number: 9024-82-2
EC Number: 232-784-7
MDL Number: MFCD00131379
Product Type: Chemical
SDS-PAGE Pyrophosphatase, Inorganic from Escherichia coli (Cat. No. I5907) was assessed on SDS-PAGE.
Unit Definition One unit will release 1.0 μmole of inorganic orthophosphate per minute at pH 9 at 25 °C.
| assay | ≥90% |
| form | lyophilized powder |
| mol wt | hexamer subunit mol wt 20 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| specific activity | ≥800 units/mg protein |
| storage temp. | −20°C |
| Application: | Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi. It plays an important role in protein, RNA, and DNA synthesis. |
| Application: | Pyrophosphatase, inorganic from Escherichia coli has been used in assay for conjugation of ubiquitin and ubiquitin-like proteins. It has been used for one-pot three-enzyme system for synthesis of Lewis x and sialyl Lewis x antigens. |
| Application: | Pyrophosphatase, Inorganic from Escherichia coli has been used as a component of transcription buffer. |
| Biochem/physiol Actions: | Pyrophosphatase from E coli (E-PPase) is an essential enzyme in yeast and bacteria The active site residues are crucial for binding to magnesium. |
| General description: | Pyrophosphatase from E coli (E-PPase) has a broader pH optimum and contains four divalent cations per subunit. It comprises 175 amino acids with additional aspartate residue in the active site cavity. Structurally E-PPase is a homohexamer with six identical 20 kDa subunits. Magnesium is a cofactor for E-PPase. |
| Other Notes: | A homohexameric protein containing 175 amino acid residues per subunit, its activity is Mg2+ dependent. It is a relatively thermostable protein. |
| Other Notes: | One unit will release 1.0 μmole of inorganic orthophosphate per minute at pH 9 at 25 °C. |
| Packaging: | 1 mg in glass bottle |
| Physical form: | Lyophilized powder in Tris-buffered salts containing protease inhibitors |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 - H335 |
| Precautionary statements | P261 - P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% |
| activity | specific activity: ≥800 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.6.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |