Monoclonal Anti-Bovine IgM antibody produced in mouse
SIGMA/I6137 - clone BM-23, ascites fluid
MDL Number: MFCD00164546
Product Type: Chemical
ELISA Monoclonal Anti-Bovine IgM antibody produced in Mouse, Clone: BM-23 (Cat. No. I6137), followed by 1:500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect Bovine IgM by indirect ELISA. Reactivity is observed with Bovine IgM but not with Bovine IgG, Human IgG, Human IgM.
| antibody form | ascites fluid |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | BM-23, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: 1:4,000 | |
| western blot: suitable |
| Application: | Monoclonal Anti-Bovine IgM antibody is suitable for use in indirect ELISA (1:1,000), western blot, immunoprecipitation and ELISA. |
| Biochem/physiol Actions: | IgM plays a key role in engulfing of the of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Bovine IgMs are glycoprotein antibodies that regulate antigen binding during immunological responses. These antibodies have three isotypes, namely, IgMa, IgMb and IgMc. Structural restraints on movement enhance the complement-fixing functions of bovine IgMs . Monoclonal Anti-Bovine IgM antibody detects an epitope on the heavy chain of bovine IgM. The antibody reacts with bovine serum, but does not react with sera from human, baboon, marmoset, gibbon, rhesus, hamster, rabbit, goat, pig, dog, rat, turkey, chicken, or catfish. Furthermore, the product does not bind to native or reduced bovine light chains. |
| General description: | Monoclonal Anti-Bovine IgM (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. IgM is highly conserved antibody in vertebrates and is expressed early during immune response. It exists as pentamer and is secreted by peritoneal B cells. |
| Immunogen: | Purified bovine IgM |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |