Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse
SIGMA/I6760 - clone HP-6017, ascites fluid
Synonym: Monoclonal Anti-Human IgG (Fc specific); Anti Human Igg Monoclonal Antibody; Anti-Human Fc Monoclonal Antibody; Anti-Human Fc Monoclonal Antibody - Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse; Mouse Anti-Human Igg
Product Type: Chemical
ELISA Monoclonal Anti-Human IgG (Fc specific) antibody produced in Mouse, Clone: HP-6017 (Cat. No. I6760), followed by 1:500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect Human IgG4 (Fc specific) by indirect ELISA. Reactivity is observed with Human IgG but not with: Human Fab, Human IgA, Human IgM and Human Kappa+Lambda.
| antibody form | ascites fluid |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | HP-6017, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rabbit, sheep, horse (IgG), goat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:3,000 |
| Application: | Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse has been used in: • enzyme linked immunosorbent assay (ELISA) • imprint immunofixation (IIF) • immunofluorometric assay (IFMA) • hemagglutination (HA) • hemagglutination inhibition (HAI) • particle counting immunoassay(PACIA) |
| Biochem/physiol Actions: | Although the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibody directed to Fc) attachment, passage through the placental membrane and protein A binding. A certain population of lymphocytes also possess a "Fc receptor". These functions indicate the importance of immunoreagents specific for the Fc fragment of human IgG. |
| Biochem/physiol Actions: | The antibody is specific for the Fc portion of human IgG and recognizes an epitope common to all human IgG subclasses. This antibody was adopted as an Fc specific reagent in the IUIS/WHO study. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Human IgG (mouse IgG2a isotype) is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins (Igs) belongs immunoglobulin super-family and have two heavy (H) and two light (L) chain, held together by disulphide linkages. The four classes of IgG can be IgG1, IgG2, IgG3 and IgG4. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. |
| Packaging: | 0.2, 0.5 mL in glass bottle |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |