Anti-IRE1α antibody produced in rabbit
SIGMA/I6785 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-ERN1; Anti-Endoplasmic reticulum to nucleus signaling 1; Anti-IRE1; Anti-IRE1P; Anti-
Product Type: Chemical
Immunoprecipitation Rabbit Anti-IRE1α (Cat. No. I6785) was used to immunoprecipitate IRE1α from Lysate of HEK-293T cells overexpressing human IRE1α. Detection antibody: Non-related Anti-IRE1α antibody. Lanes 1. 2 μL antibody 2. 5 μL antibody 3. Immunoblotting positive control: total Lysate of HEK-293T cells overexpressing human IRE1α
Immunoblotting Whole extract of HEK-293T cells overexpressing human IRE1α was separated on SDS-PAGE and probed with Rabbit Anti-IRE1α (Cat. No. I6785). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody 3. 2 μg/mL antibody + IRE1α immunizing peptide (human, 953-969)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~110 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-5 μg using lysate of HEk-293T cells expressing human IRE1α |
| indirect immunofluorescence: suitable | |
| western blot: 1-2 μg/mL using whole extract of HEK-293T cells expressing human IRE1α | |
| UniProt accession no. | O75460  |
| Application: | Anti-IRE1α antibody produced in rabbit has been used in western blotting and immunoprecipitation. |
| Biochem/physiol Actions: | ER transmembrane kinase/endoribonuclease serves as an ER stress sensor that initiates the splicing of the mRNA encoding X-box–binding protein 1 (XBP-1) upon activation. Spliced XBP-1 mRNA produces homeostatic transcription factor XBP1. Under ER stress, the RNase activity of IRE1α cleaves many ER-localized mRNAs associated with apoptosis. The cytosolic motif of the activated form of IRE1α associates with the adaptor protein TRAF2 (tumor necrosis factor (TNF)–associated factor 2). This complex activates the c-Jun N-terminal kinase (JNK) signaling pathway. |
| Biochem/physiol Actions: | Inositol-requiring enzyme-1 (IRE1) is involved in the unfolded protein response (UPR), a transcriptional program induced by endoplasmic reticulum (ER) stress. The endonuclease activity of IRE1 autoregulates its mRNA and is required for the UPR. IRE1 senses the status of luminal protein folding in the ER via its N-terminal luminal domain. Presence of unfolded and misfolded proteins leads to dimerization, trans-autophosphorylation and activation of IRE1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Inositol-requiring enzyme-1 (IRE1), consists of an N-terminal endoplasmic reticulum (ER) luminal domain, a transmembrane domain, and a C-terminal cytoplasmic region composed of a Ser/Thr protein kinase domain and a site-specific endoribonuclease (RNase) domain. |
| General description: | The gene ERN1 (endoplasmic reticulum to nucleus signaling 1) encodes an ER transmembrane kinase/endoribonuclease that is also referred to as IRE1α (inositol-requiring transmembrane kinase and endonuclease 1α). The serine-threonine protein kinase encoded by this gene is ubiquitously expressed in cells and tissues. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |