IgM from human serum
SIGMA/I8260 - reagent grade, ~95% (HPLC), buffered aqueous solution
Synonym: Human IgM
MDL Number: MFCD00163928
Product Type: Chemical
Illustration of IgM
| assay | ~95% (HPLC) |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| grade | reagent grade |
| Quality Level | 300  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Application: | Purified human IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. IgM from human serum was used as standard in ELISA (100 μg/ml) and dot blot assay. Antibody concentration range of 200 μg/ml to 1 mg/ml was used in IgM binding assays. IgM from human serum has been used • in ELISA inhibition assay • in immunoblotting • as MW (molecular weight) standards • in IgM binding assay • to confirm the depletion of immunoglobulins |
| Biochem/physiol Actions: | Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | There are five immunoglobulin classes in humans. Out of these, immunoglobulin M (IgM) is a high molecular weight protein that has five or six subunits. IgM monomers are made of two heavy chains and two light chains connected by a disulfide bond. Human serum shows low concentration of IgM monomers. It has a high carbohydrate content of about 12%. It is the first immunoglobulin produced by neonates. IgM antibodies are present as pentamers in the serum and are produced in response to antigens. IgM from human serum is purified from normal human serum by precipitation and gel filtration techniques. The immunoglobulin is determined to be atleast 95% pure by HPLC procedures. |
| Packaging: | 1, 5 mg in glass bottle |
| Packaging: | 10 mg in serum bottle |
| Physical form: | Solution in 0.05 M Tris-HCl, 0.2 M sodium chloride, pH 8.0, containing 15 mM sodium azide. |
| Preparation Note: | Store at 2-8 °C. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ~95% (HPLC) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |